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HomeProductsFluorescence SpectrometersBio-applications: FP-8300

JASCO FP-8300 Fluorescence Spectrometer

The JASCO FP-8300 is a versatile model, specifically designed for bio-applications, such as stopped flow, fluorescence anisotropy and auto-titration applications. 

jasco-j1100

In Brief

 

Wavelength range on both Ex and Em 200 - 750 nm (900 nm, option)

Automatic cut-off filter

High sensitivity (8000:1 (RMS), band width 5 nm)

High resolution of 1.0nm

Installation optional

Simplicity and Ease-of-Use

The JASCO FP-8300 fluorescence spectrometer has two intuitive interfaces allowing even first time users to collect data with confidence.

The intelligent remote module (iRM)

with a colour LCD touch screen & USB storage

OR

PC control with Spectra

ManagerTM II software.


See the Spectra manager



Standard measurement programs for both interfaces:

  • Spectra measurement: 5 different types. Luminescence, emission, excitation, synchronous and single beam.

    Quantitative analysis: two photometric modes and three quantitation methods.

    Absorption-transmission: Measurement of transmission or absorbance (requires FUV-803 cell block.

  • Time course: Measurement of temporal changes at a fixed wavelength with a choice of time intervals.

    Fixed wavelength: Measurement of fluorescence at up to four Ex and Em wavelengths.

    3-D spectra: This function can be used to graphically display the fluorescence characteristics of the sample.

 Features of JASCO FP-8300 Fluorescence Spectrometer

  • Auto gain
    The signal/noise is optimised throughout the scan range by using the Auto-Gain which automatically adjusts the gain due to fluorescence intensity.

    Auto sensitivity control
  • This function of JASCO Fluorescence Spectrometer allows the creation of a calibration curve for a wide concentration range without changing the measurement parameters.

    Auto cut off filter
  • This enables spectra to be obtained without the peaks that originate from higher order diffracted light.

    Phosphorescence measurements
  • A high-speed chopper for the FP-8300 offers phosphorescence spectrum measurements as well as advanced phosphorescent lifetime and quantitative analysis measurements. The liquid nitrogen sampling accessory can be added for additional flexibility.

 

Luminescence spectra of cooled Benzene measured with 255 nm excitation at 77 K. The blue trace in the figure is the normal emission spectrum; the red trace is the phosphorescence spectrum measured using a 5 msec delay.

Wide range of optional accessories for JASCO FP-8300 Fluorescence Spectrometer

The FP-8300 can be expanded to accept any optional accessories such as the automatic titration unit or microplate reader. Using FP-8300, the most suitable system can be configured for example to measure phosphorescence of samples or for quantum yield determination using the integrating sphere unit.

See the Full range of Accessories.

Contact us for more information on the JASCO FP-8300 Fluorescence Spectrometer.

 

 

 

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