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UV Vis Spectrophotometers

UV Vis SpectrophotometersJASCO V-750 UV-Vis Spectrophotometer

Ultraviolet visible spectrophotometers measure how much light of a given wavelength passes through a sample, and how much is absorbed to provide information about molecular composition, concentration, structure or activity. These instruments focus on the UV and visible wavelength ranges of light, typically 190 to 1100 nm. The resulting peaks in a spectrum can indicate if a sample is mainly composed of organic material such as aromatics, alcohols, polymers and esters, or of biomolecules like nucleic acids and proteins.

Key factors to consider when looking to purchase a UV Vis spectrometer include the optical configuration, detector type, the band width and stray light level.

Read more: UV Vis Spectrophotometers

FTIR Spectrometers

Jasco FT/IR-4000 Series

Fourier transform infrared spectrometers measure the amount of IR radiation that absorbed or transmitted by the sample. The results are plotted in a spectra representing a molecular ‘fingerprint’ that analysts can use to identify and quantify material. Various functional groups found in polymers and organic compounds can be identified because of the similar absorption frequencies for those groups in different molecules.

FTIR spectroscopy is particularly useful for liquid samples such as milk and wine. In dairy applications FTIR can be used to measure parameters such as fat, protein and lactose content in milk. For wine, the content of multiple parameters such as sugars, acids and ethanol can be measured. Read more: FTIR Spectrometers

Digital Polarimeters

 Jasco P2000 Polarimeter

Polarimetry measures the rotation of polarized light as it passes through an optically active fluid to determine the molecular structure, concentration and purity of an organic or inorganic compound.
Optical activity is a description given to materials which can bend polarised light and is a property unique to chiral substances. Examples of optically active fluids are sugars, peptides and volatile oils.

Optical activity is influenced by temperature, the wavelength of the light, and the length of the optical path. The longer the path of the light through an optically active substance, the larger the angle of rotation. Read more: Digital Polarimeters

Fluorescence Spectrometers

Jasco FP-8300 Fluorescence Spectrometer

A Fluorescence spectrometer or fluorometer, measures the intensity of light or photons emitted (emission spectrum) from a sample after it has absorbed photons (excitation spectrum). When activated by a light source, typically a Xenon lamp, the intensity of the emission allows the concentration of an analyte in solution to be determined. In general molecules that fluoresce have one or more aromatic groups in their structure. Some molecules fluoresce naturally, and others must be modified to fluoresce. By measuring changes in fluorescence intensity analysts can probe structural changes or binding of two molecules. Fluorescence can be used to investigate real-time structure and dynamics both in solution state and under microscopes, particularly for bio-molecular systems. The wavelength of tryptophan fluorescence for example can be used to determine whether a tryptophan is in an aqueous solution (longer wavelength) or deep within the protein (shorter wavelength). Read more: Fluorescence Spectrometers

Raman Spectrometers

 Jasco NRS 4500 Spectrophotometer

Raman spectroscopy provides information about molecular vibrations that can be used for sample identification and quantitation. Raman spectroscopy is complementary to IR absorption spectroscopy and involves shining a monochromatic light source (i.e. laser) on a sample and detecting the scattered light.

Raman spectroscopy provides a fingerprint by which the molecule can be identified. It can be used to study polymorphic forms of compounds such as Active Pharmaceutical ingredients (APIs) and to study changes in chemical bonding and symmetry of molecules.

Read more: Raman Spectrometers

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